Seminar Details

Seminar Details


Monday

Nov 23

3:30 pm

A Primer on Mass Spectrometry Based Proteomics

Dave Goodlett

Seminar

University of Washington - Department of Medicinal Chemistry

I'll review the basics of peptide/protein chemistry pertinent to sequencing by MS and discuss how the MS instruments produce spectra which are "converted" to sequence by software as well as some about the software. So, 1/3 each of 1) protein chemistry (and the why of how we do proteomics with MS), 2) a description of fragmentation mechanisms (this is what people casually refer to as sequencing) and 3) the vagaries of finally sequence assignment to the raw data.